E-Plate Inserts are single use, disposable devices used for performing cell-cell interaction assays on the RTCA DP, SP, MP or iCELLigence Instruments. The E-Plate Insert comprises a plate cover (lid), the insert device itself, and a lower receiver plate (Figure 1). The insert device has 16 wells that are sealed at the bottom with a microporous polyethylene terephthalate (PET) membrane. The median pore size of the membrane is 0.4μm. The receiver plate has 16 wells, each of which serves as a reservoir for media to pre-equilibrate the insert device and cells prior to assembly in an E-Plate.