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Langendorff Apparatus

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The Langendorff Apparatus & Thermostat Controller provides a complete, self-contained and thermostatically-controlled unit for the perfusion of small animal hearts. Two separate perfusate reservoirs are constantly oxygenated and kept at a constant temperature. The solutions pass through tubes around the Peristaltic Pump (regulated by the STH Pump Controller) in order to pressurize them.
The pressurized perfusate solutions are re-warmed when they re-enter the bath unit by coiled tubes and then feed into the heated junction block, from which the cannulated heart is attached. Perfusate is directed towards the junction block, however only one is used at a time to perfuse the heart with the other redirected via the 3-way valve back to the reservoir. This allows an easy change between the two reservoirs and prevents the formation of air bubbles or entry of air into the heart upon reservoir change. The heated junction block ensures the perfusate is maintained at a constant temperature prior to entering the heart.
The system is best suited for use with small animal hearts such as mouse, rat and guinea pig. For a complete research system visit the Langendorff System - Panlab page. Alternatively, the Langendorff System - Radnoti page offers a comprehensive solution.

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