Replica Plating Device utilizes a locking ring which secures sterile velveteen squares onto a PVC cylinder for easy control of the plating device. To produce replicas, the petri dish carrying microbial colonies is inverted and lowered onto the cloth surface and then lifted. Then a fresh sterile nutrient plate is lowered onto the velveteen surface and lifted, leaving a replica of the original colonies in the new dish.The replica plating device may be disinfected between uses with a brief rinse in ethanol or chlorine bleach. The aluminum ring is 102mm diameter and the PVC cylinder is 69mm high. For use with 90 to 100mm petri dishes. One bag of 12 Velveteen Squares is included.