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Safranine O

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Safranine o or basic red 2 is used as a counter stain in some staining protocols, coloring all cell nuclei red. This is the classic counter stain in a gram stain. It can also be used for the detection of cartilage, mucin and mast cell granules.

Features:

Cas
no.

477-73-6

C. I. No.

50240

Chemical
formula

c20h19cln4

Molecular
weight

350. 85

Dye
content

~95%

Absorption
max (etoh 50%)

530 -
534 nm

Absorptivity
(1%,1cm)

>
1350 (on dry basis)

Loss
on drying (110° c)

8% max

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